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This study was conducted to establish the tissue culture system for Korean domestic Miscanthus sinensis, which is used in various purposes such as forage, and bio-energy resources. With the mature seed of Miscanthus, optimum concentrations of plant growth regulators were identified for an efficient callus induction and regeneration. Among the treatments of 1~10 2,4-D, IBA, or NAA, callus induction rate was highest (85.3%) on MS medium containing 5 2,4-D. Under the condition, the callus were efficiently induced and proliferated with comparably lower frequencies of callus browning. In shoot regeneration, the treatment of NAA combined with BAP seemed to contribute more efficient conditions to shoot regeneration than those of NAA with Kinetin or 2-iP. Especially, regeneration efficiency and number of regenerated plants were 83.7% and 5.5 in 3 NAA with 5 BAP, respectively, which were higher frequencies than those in NAA with Kinetin or 2-iP. In results, 5 2,4-D and 3 NAA combined with 5 BAP were efficient for embryogenic callus induction and regeneration of Miscanthus. This system would be useful for mass-propagation and developing new cultivars via tissue culture of Miscanthus sinensis.

Establishment of Callus Induction and Plant Regeneration System from Mature Seeds of Miscanthus sinensis