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Luffa  is a kind of melon crop widely cultivated in temperate regions worldwide. Browning is one of the serious factors affecting the quality of  Luffa . Therefore, the molecular mechanism of  Luffa  browning is of great significance to study. However, the molecular diversity of  Luffa  cultivars with different browning-resistant abilities has not been well elucidated. In our study, we used high-throughput sequencing to determine the transcriptome of two  Luffa cylindrica  cultivars ‘2D-2’ and ‘35D-7’. A total of 115,099 unigenes were clustered, of which 22,607 were differentially expression genes (DEGs). Of these DEGs, 65 encoding polyphenol oxidase, peroxidase, or ascorbate peroxidase were further analyzed. The quantitative real-time PCR (RT-qPCR) data indicated that the expression levels of the  LcPPO  gene (Accession No.: Cluster-21832.13892) was significantly higher in ‘35D-7’ compared with that in ‘2D-2’. Several  POD  genes (Accession No.: Cluster-21832.19847, Cluster-21832.30619 and Cluster-48491.2) were also upregulated. Analysis of the plantTFDB database indicated that some transcription factors such as WRKY gene family may also participate in the regulation of  Luffa  browning. The results indicated that the divergence of genes expression related to enzymatic reaction may lead to the different browning resistances of  Luffa . Our study will provide a theoretical basis for breeding of browning-resistant  Luffa .

Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica