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Management of insecticide resistance in the mosquito CuZex Pipiens complex is important because this complex is a major vector of lymphatic filariasis and West Nile fever, which occurs worldwide, and has been exposed to frequent chemical controls with a variety of insecticides, We applied the SNaPshot assay, a primer extension-based genotyping method, to the identification of both insecticide resi$tance-associated point rnutations at insecticidal active sites and the mosquito subspecies distributed in Japan, Variations at LeulO14 and VallO16 positions in the sodium channel and Serl19 in Drosophila Ace-paralogous acetylcholiesterase (p-Ace) were individually tested for the detection of mutations conferring decreased sensitivity to nerve insecticidal targets. Moreover, sequence differentiations i Drosophita Ace-ortholegous acetylcholinesterase {o-Ace) gene were concomitantly tested for the detection of subspecies of Cx, Pij)iens Patgens, Cx. p. molestus and Cx. quinquojinsciatus. The exactness of the SNaPshot assay was verified using standard laboratory strains and mutagenized synthesized DNA. The assay was then applied to laboratorycolonized mosquitoes that were captured from Kyushu and Honshu, Japan. Judgments for both Cx, P, m, and Cx, P. Pallens by SNaPshot assay agreed with the respective autogenicities of the colonies tested, except for an erroneous individual. Mutation of LeulO14 to Phe and Ser in the sodium channel genes were specifically identified in the samples of Cx. p. m. and Cx. P. Pallens., respectively. No mutation was detected for the other target sites.

プライマー伸長に基づくジェノタイピングによるアカイエカ種群蚊の亜種と殺虫剤抵抗性遺伝子の同時識別