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To develop a simple, precise and accurate UV-method with multivariate calibration technique for estimation of rilpivirine hydrochloride in pharmaceutical dosage form. This technique is based on the use of the linear regression equations by using relationship between concentration and absorbance at five different wavelengths like 301, 303, 305, 307 and 309 nm. The results were treated statistically and were found highly accurate, precise and reproducible. The rilpivirine hydrochloride shows absorption maxima at 305 nm and obeyed Beer’s law in the range of 0.5-3.0 μg/mL. The % recovery of tablets was found to be in the range of 101.1-101.7%. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.358 and 1.086 μg/mL, respectively. The low % RSD values are indicates the accuracy and precise of the method. The proposed methods can be successfully applied for method development, validation and multivariate analysis of rilpivirine hydrochloride.

UV-Visible Spectrophotometric Method for the Estimation of Rilpivirine Hydrochloride in Pharmaceutical Dosage Form by Using Multivariate Technique