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To enhance Bacillus thuringiensis (Bt) efficacy, four Cry toxins were purified from four differentBt strains and assessed in their combined efficacy. The Cry mixtures significantly expanded their target insectspectra. Bacterial culture broth of Xenorhabdus nematophila (Xn) significantly suppressed insect cellularimmune response and increased Cry toxicity. The addition of Xn culture broth to Cry mixture significantlyenhanced Bt efficacy in target insect spectrum and insecticidal activity. Key words Cry toxin, immunosuppression, Bacillus thuringiensis, Xenorhabdus nematophila          .        ,      (Beckage, 2008).        .       .  ,       (Gillespie et al., 1997).          (Park and Lee, 2012).                (Adamo, 2008).(Bacillus thuringiensis)        (Crystal: Cry)  (Roh et al., 2007). Cry   ,     (Bravo et al., 2012).  700  Cry   (Crickmore et al., 2014),     40%     70    Cry (Cry1, Cry2 ) ,  70%   (Cry1A, Cry1B ) ,  70-95%    (Cry1Aa, Cry1Ab )  (Crickmore et al., 1998). Cry     130 kDa    65-70kDa       ,      N ()  N C ( )   60 kDa      (de Magd et al., 2001).       . Cry1Ab     (Kd=1 nM)     Cry   domain I    α    Cry    .

A Technique to Enhance Insecticidal Efficacy Using Bt Cry Toxin Mixture and Eicosanoid Biosynthesis Inhibitor