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We was investigated parasporal inclusion proteins change to use industrial medium of new strain Bacillus thuringiensis CAB 565, CAB 566. To confirm medium’s oxygen efficient consist of glucose and yeast extract, we was conducted oxygen transfer coefficients (KLa) of medium’s concentration and impeller in 20 l-Jar fermentor. When to increase air flow rate and medium concentration, KLa rate is rise. Also it is effective on agitation rate 200 rpm, but KLa rate is decrease when to rise agitation rate. To hold dissolved oxygen rate (upper 50%), Air flow rate is steadily increase on culture to use microsparger. When 16 hour of culture stage, B.t. CAB 565 and B.t. CAB 566 harvested respectively 2.3 × 10, 1.8 × 10 viable cell/ml. When 54 hour, B.t. CAB565, 566 harvested respectively 1.9 × 10, 1.4 × 10 spore/ml. To resulting carbon’s concentration, It is the most effective that glucose concentration is contained 5% in medium.

Effect on the Production of Toxin Protein of Bacillus thuringiesis by Culture Conditions