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This study was performed to investigate pre-harvest residue limit (PHRL) in amaranth, toestimate biological half-life of emamectin benzoate and identify the characteristics of the residue. Pesticidesof standard and double appplication rate, were sprayed once on amaranth at 0, 1, 2, 3, 5, 7, 10 ,14 daysbefore harvest. Amaranth sample was extracted with acetonitrile and partitioned with dichloromethane, andpesticide residues were determined with LC/MS/MS. The limit of detection of emamectin benzoate was 0.01mg/kg. Recoveries of emamectin benzoate (B 1a , B 1b ) at two fortification levels of 0.1 and 0.5 mg/kg, B 1a were93.3 ± 0.7% and 93.2± 7.7%, B 1b were 106.6 ± 1.9% and 80.5± 6.6%, respectively. The biological half-livesof emamectin benzoate were about 2.0 days at standard application rate and 1.7 days at double applicationrate, respectively. The PHRL of emamectin benzoate were recommended as 0.84 mg/kg for 10 days beforeharvest.Key words Amaranth, Emamectin benzoate, Pesticide residue, Pre-harvest residue limit

Establishment of Pre-Harvest Residue Limit (PHRL) of Emamectin benzoate during Cultivation of Amaranth