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Environment-friendly agro-materials are are likely to be preferred to chemical insecticides recently. For this reason, many studies are conducted to develop environment-friendly insecticides containing natural materials. This study was also conducted so as to assess ecotoxicity for Emulsifiable concentrate (EC) containing 30% of garlic extract or two plant essential oils (Zanthoxylum, Lemongrass) expected to prevent from pests and be used for agro-materials. Target species used to assess acute toxicity were invertebrate (Daphina magna), fish (Oryzias latipes), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The EC50 values for of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC to Daphina magna were 3.3, 10, and 10 mg L 1 , respectively. The category of garlic extract 30% EC was moderately toxic, while those of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were slightly toxic according to standard of USEPA. EC50 for both of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than 10 mg L 1 then they were considered as slightly toxicity. In case of acute toxicity test to fish, LC50 of garlic extract 30% EC was 3.3 mg L 1 . Zanthoxylum oil 30% EC and lemongrass oil 30% EC indicated LC50 >1 0 mg L 1 . Classification of acute toxicity to all test substances was in Korea criteria. Acute contact and oral toxicity test to Honeybee were conducted. As a result, LD50 of all test substances were more than 100 a.i. µg bee 1 in the acute contact test while LD50 of garlic extract 30% EC was 4.4 a.i. µg bee 1 and LD50 of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than 100 a.i. µg bee 1 . In case of acute toxicity test to earthworm, LC50 of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC were 267, 592, and 430 mg kg 1 , respectively. In conclusion, if the safety for earthworm is confirmed, these substances are expected to be use for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.

Acute Ecotoxicity Evaluation of 3 Emulsifiable Concentrates Containing Garlic Extract, Zanthoxylum Extract, and Lemon Grass Oil Originated from Plant