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Pdgfra -expressing ( Pdgfra+ ) cells have been implicated as progenitors in many mesenchymal tissues. To determine lineage potential, we generated  Pdgfra rtTA   knockin mice using CRISPR/Cas9. During lung maturation, counter to a prior study reporting that  Pdgfra + cells give rise equally to myofibroblasts and lipofibroblasts, lineage tracing using  Pdgfra rtTA ;tetO-cre  mice indicated that ~95% of the lineaged cells are myofibroblasts. Genetic ablation of  Pdgfra +   cells using  Pdgfra rtTA  -driven diphtheria toxin (DTA) led to alveolar simplification, demonstrating that these cells are essential for building the gas exchange surface area. In the adult bleomycin model of lung fibrosis, lineaged cells increased to contribute to pathological myofibroblasts. In contrast, in a neonatal hyperoxia model of bronchopulmonary dysplasia (BPD), lineaged cells decreased and do not substantially contribute to pathological myofibroblasts. Our findings revealed complexity in the behavior of the  Pdgfra -lineaged cells as exemplified by their distinct contributions to myofibroblasts in normal maturation, BPD and adult fibrosis.

Pdgfra marks a cellular lineage with distinct contributions to myofibroblasts in lung maturation and injury response