English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The internal  N  6 -methyladenosine (m 6 A) methylation of eukaryotic nuclear RNA controls post-transcriptional gene expression, which is regulated by methyltransferases (writers), demethylases (erasers), and m 6 A-binding proteins (readers) in cells. The YTH domain family proteins (YTHDF1–3) bind to m 6 A-modified cellular RNAs and affect RNA metabolism and processing. Here, we show that YTHDF1–3 proteins recognize m 6 A-modified HIV-1 RNA and inhibit HIV-1 infection in cell lines and primary CD4 +  T-cells. We further mapped the YTHDF1–3 binding sites in HIV-1 RNA from infected cells. We found that the overexpression of YTHDF proteins in cells inhibited HIV-1 infection mainly by decreasing HIV-1 reverse transcription, while knockdown of YTHDF1–3 in cells had the opposite effects. Moreover, silencing the m 6 A writers decreased HIV-1 Gag protein expression in virus-producing cells, while silencing the m 6 A erasers increased Gag expression. Our findings suggest an important role of m 6 A modification of HIV-1 RNA in viral infection and HIV-1 protein synthesis.

N 6 -methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression