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The objective of this study was to examine the effect of the polymorphism in the promoter oftoll-like receptor 4 (TLR4) on mastitis and milk production traits. Two novel single nucleotidepolymorphisms (SNPs) G-1539A before 5’-upstream region and G+265C in the 5’-UTR of TLR4gene were detected, and genetic association analysis was applied on 421 Chinese Holsteincows. The statistical analysis indicated that two SNPs was significantly associated with 305Days milk yield (P<0.05) and somatic cell score (SCS) respectively (P<0.01). Our results provideevidence that polymorphisms in the promoter of TLR4 are associated with milk productiontraits and SCS, and may be used as a possible candidate for marker-assisted selection andmanagement in Holstein cattle breeding program

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Polymorphism in the promoter of &amp;lt;i&amp;gt;TLR4&amp;lt;/i&amp;gt; gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein