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Identifying sources of renewable energy is extremely important. The potential of Tetraselmis sp. in biodiesel production was investigated using a completely randomized design with four treatments. Tetraselmis sp. was cultured on media containing different concentrations of nitrate there are 2 mM and 15 Mm and s 25 ‰ and 35 ‰ salinity. The treatments namely are (N2S25), (N2S35), N15S25) and (N15S35). Analysis of the fatty acid content using gas chromatography-mass spectrometry (GC-MS). The results showed that the highest growth occurred in culture that contained 15 mM of nitrate and 35 ‰ salinity. The lowest growth occurred in culture containing 2 mM of nitrate and 25‰ of salinity. The highest lipid content was found in cultures containing 2 mM of nitrate and 25‰ salinity, it is 34.83%. Seven fatty acids were detected in culture containing 2 mM of nitrate and 25‰ salinity namely myristic acid (4.02%), palmitic acid (40.59%), palmitoleic acid (29.06%), stearic acid (0.95%), oleic acid (12.52%), gamma-linolenic acid (2.56%), and arachidonic acid (9.38%). Four fatty acids palmitoleic acid (8.99%), palmitic acid (37.34%), oleic acid(44.89%), and stearic acid (8.78%) were detected in 2 mM of nitrate with 35‰ salinity. The fatty acids have potential to be used as raw material for biodiesel production

Effects of Nitrate and Salinity on Fatty Acid Composition of Marine Tetraselmis sp.: Potential as Biodiesel