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The present study highlights the utilization of wastes such as cowpea outer pod generated from agro industries for laccase production using Myrothecium gramineum LCJ177 under solid-state fermentation. Conventional methods were used to optimize the process parameters. The classical one-factor-at-a-time method showed that the optimal starch concentration was 1 g/L, peptone concentration was 0.5 g/L, copper sulfate concentration was 0.6 mM, and pyrogallol concentration was 0.8 mM. Likewise, the suitable physical conditions were an initial pH of five of the culture medium, the temperature of 30°C and moisture content of 60%. Utilization of dried cowpea outer pod as a substrate reduces the pollution levels by converting agro-wastes as useful by-products. ARTICLE INFO Article history: Received on: February 13, 2018 Accepted on: September 11, 2018

Laccase production by Myrothecium gramineum and its optimization under solid state fermentation using cowpea pod as substrate