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The study was performed to check the chemical, antioxidant, antimicrobial and protein binding qualities of the hydro-ethanolic extract of Celastrus paniculatus (CP). Hydro-ethanolic extract of CP exhibited low antibacterial and antifungal activities. Excellent antioxidant activities (60-80 %) were noticed for various antioxidant assays viz DPPH (2,2-diphenyl-1-picrylhydrazyl), MC (Metal chelating), FRAP (Ferric reducing), SA (Superoxide radical), and NO (Nitric oxide). Total phenolic content (TPC) and total flavanoids content (TFC) of the extract was 1.38Â±0.15 mg/g (expressed in equivalents to gallic acid) and 8.91Â±0.21 mg (expressed in equivalents quercetin) respectively. Moreover, at p ≤ 0.05, significant linear correlation was noticed between the TFC/TPC and antioxidant activities favoured which highlights the medicinal property of CP. Protein binding assay was performed and an observed binding constant (1.11Â±0.92A—10-5 ÂµM-1) was almost close to control (aspirin). Anti-inflammatory assay revealed potent anti-inflammatory activity of hydro-alcoholic extract of CP. These significant findings of CP may provide compelling scientific evidence of its therapeutic potential.

Determination of phytochemical, antioxidant, antimicrobial, and protein binding qualities of hydroethanolic extract of Celastrus paniculatus