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Tepi town is rich in lignocellulosic wastes such as coffee wastes, sawdust and sugarcane bagasse which possibly used as mushroom substrate. Therefore the purpose of this study was to assess the suitability of three locally available substrates (coffee waste, saw dust and sugarcane bagasse) and their combinations for cultivation of P. ostreatus. Spawn was prepared with sorghum supplemented with 5%wheat bran and 1% gypsum. Main substrates were weighed, soaked in water and steam sterilized for 1 h in plastic bags and inoculated with fully grown spawn. All inoculated bags were arranged in completely randomized design on shelves in mushroom growing room at ambient temperature (23 ± 2°C). Following, days taken for complete mycelial colonization, pinhead formation, fruit body formation and maturation, biological efficiency (BE), stipe length and pileus diameter of mature mushroom were recorded and analyzed using SPSS version 16. Majority of substrate showed significant variation in days taken for mycelial colonization, pinhead formation and biological efficiency (BE) at p≤0.05 whereas incubation periods between flushes, stipe length and pileus diameter were not significantly varied. Despite lignocellulosic waste being a menace to the environment, represent a potential bioresource for production of oyster mushrooms.

journal of applied biology and biotechnology

Substrate optimization for cultivation of Pleurotus ostreatus on lignocellulosic wastes (coffee, sawdust, and sugarcane bagasse) in Mizan–Tepi University, Tepi Campus, Tepi Town