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The present study aims to determine the location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi associated with pea seeds. The pea seeds categorized (asymptomatic, moderately discolored, and shrivelled discolored seeds) and seeds of each category were assayed for the presence of bacterial pathogen P. syringae pv. pisi. Seed samples carrying high incidence (89.5% and 91.34%) of the bacterial pathogen were used for the microtome sectioning individually. It was observed that the cells of P. syringae pv. pisi were found in large numbers in radicle and hilum region and space between seed coat and spermoderm in shrivelled discolored seed category while in moderately discolored seed category bacterial cells were less in numbers and found into the inner side of seed coat and endosperm. Bacterial colonization within seed tissue caused necrosis, formation of lytic cavities, and reduction in cell contents.

Location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi carried by pea seeds