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This study was conducted to evaluate the characteristics of dark fermentative H2 production from microalgae (Chlorella vulgaris) using batch reactors under mesophilic (25, 35°C) and thermophilic (45, 55°C) conditions. The H2 yield and H2 production rate increased with increasing temperature. The maximum H2 yield and H2 production rate were 56.77 mL H2/g dcw, 3.33 mL H2/g dcw·h at 55°C, respectively. The activation energy calculated using Arrhenius equation was 36.24 kcal/mol, which was higher than that of dark H2 fermentation of glucose by anaerobic mixed culture. Although the concentration of butyrate was maintained, the concentrations of lactate and acetate increased with increasing temperature. The H2 yield was linearly proportional to acetate/ butyrate ratio.

Hydrogen Production from Microalgae in Anaerobic Mesophilic and Thermophilic Conditions