Open AccessDetection of Human Cytomegalovirus in patients with Colorectal Cancer by Nested-PCR
Author(s) -
Farzaneh Tafvizi,
Zahra Tahmasebi Fard
Publication year - 2014
Publication title -
asian pacific journal of cancer prevention
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 75
eISSN - 2476-762X
pISSN - 1513-7368
DOI - 10.7314/apjcp.2014.15.3.1453
Subject(s) - nested polymerase chain reaction , colorectal cancer , human cytomegalovirus , cytomegalovirus , nested case control study , virology , medicine , cancer , oncology , polymerase chain reaction , biology , virus , herpesviridae , case control study , genetics , viral disease , gene
The association of colorectal cancer with human cytomegalovirus (HCMV) is a controversial issue in cancer research. This study aimed to identify the HCMV virus in colorectal cancer tissues and to investigate the association of HCMV with colorectal cancer. In this study, 50 cancer tissue samples and 50 samples without colon cancer were studied in order to identify the HCMV virus through nested-polymerase chain reaction. The virus was identified in 15 cases of colorectal cancer tissues (15/50) and in 5 cases of normal tissues (5/50). Eight cases of adenocarcinoma tissues were in a moderately differentiated stage, and 7 cases had well-differentiated stage tissues that were positive for viral DNA. The findings were statistically evaluated at a significance level of p<0.05. The HCMV virus could play a role in creating malignancy and the progress of cancer through the process of oncomodulation.
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