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The genetic relationships of offspring cultivars from ‘Yumeyong’ peach were evaluated by using Fourier transform infrared (FT-IR) spectroscopy. Four offspring cultivars were bred by controlled hybridization between ‘Yumyeong’ and ‘Chiyomaru’, 2 from open pollination, and 1 from bud sport mutation. The FT-IR spectrum of genomic DNA were analyzed by using principal component analysis (PCA), partial least square discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA). FT-IR spectra showed that typical spectral differences were existed in the frequency regions of guanine ring (C6 = C6), thymine ring (C4 = O4), cytosine, N-H stretching (amide I), C = O stretching vibrations (amide II), and PO2 ionized asymmetric and symmetric stretching, respectively. Among the offspring cultivars between ‘Yumyeong’ and ‘Chiyomaru’, ‘Mihong’ and ‘Misshong’ showed higher genetic similarity to ‘Yumyeong’ than ‘Chiyomaru’. On the other hand, ‘Yumi’ and ‘Soomee’ showed higher genetic similarity to ‘Chiyomaru’ than ‘Yumyeong’. ‘Wolmi’, a mutant cultivar from ‘Yumyeong’, showed close relationship with ‘Yumyeong’, however, the genetic similarity was lower compared to other crossbreeding cultivars, ‘Misshong’, and ‘Mihong’. Additional key words: discrimination system, hierarchical clustering analysis, multivariate analysis, partial least square discriminant analysis (PLS-DA), principal component analysis (PCA)

Genetic Relationship Analysis by using FT-IR Spectrum of Genomic DNA from Peach Cultivars Related to ‘Yumyeong’