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This study aimed to enhance storage and freshness of strawberry fruits and foliage vegetables by spray treatment with Chlorella vulgaris as a bio-fertilizer. The tested strain, C. vulgaris CHK0008, was isolated from an organically cultivated rice paddy and identified as C. vulgaris by its morphology and 18S rDNA and 23S rDNA sequence homology. We successfully cultured C. vulgaris CHK0008 in BG11 modified medium (BG11MM) and adjusted 2.15 × 10 6 cell/mL C. vulgaris CHK0008 to one OD value by measuring the optical density at 680 nm using a UV-vis spectrophotometer. The soluble solid content of ‘Seolhyang’ and ‘Yukbo’ strawberry fruits treated by spray application with C. vulgaris CHK0008 was enhanced by 22.2% and 11.5% respectively, compared to untreated controls. Additionally, the decay rates of treated ‘Seolhyang’ and ‘Yukbo’ strawberry fruits decreased 63.8% and 74.4% respectively, compared to untreated control. Surface color changes and chlorosis of leaves in leaf vegetables such as lettuce, kale, red ornamental kale, white ornamental kale and beet were observed in samples treated with water spray for 10 days after cold storage. However, the decay rate of leafy vegetables treated with foliar application of 25% C. vulgaris CHK0008 liquid culture was significantly decreased compared to that of the untreated control during storage at 4°C. Additional key words: freshwater algae, fresh-cut vegetable, Seolhyang, spray and soil drenching application, Yukbo *Corresponding author: ckshim@korea.kr ※ Received 16 June 2014; Revised 9 July 2014; Accepted 14 July 2014.   2014  (PJ00912702)   . C 2014 Korean Society for Horticultural Science      (wellbeing)          .              (Kim et al., 2011, 2012, 2013). ,                 (71.9%)            (Kim and Lee, 2013; Lee and Kim, 2012).                 ,    , , ,            (Lee et al., 2009).                  , , , , , , ,  873      (In and Kim, 2008; Kim et al., 1995).                      .      , ,       , 2009    19,200  (KREI, 2010).                     ,               (Hwang and Ku, 2004; Jeong et al., 1990; Park and Hwang, 2010). (Chlorella vulgaris)   (microalgae) , , ,       .  (Kang et al., 2013),  (Bai and Cha, 1997; Becker, 1994), (Park et al., 2002), (Heo et al., 2006),    (Travieso et al., 2002)         (Kim et al., 2003).               14:10h(Light:Dark)     glucose(5g・L -1 )     (plant growth regulators)  (auxin) (cytokinin)    (Stirk et al., 2014). Raposo et al.(2011)  (plant growth-promoting rhizobacteria, PGPR)   (meadow clover)            .            (C. vulgaris, CHK0008)                .

Effect of Chlorella vulgaris CHK0008 Fertilization on Enhancement of Storage and Freshness in Organic Strawberry and Leaf Vegetables