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identified to genus, but has been identified as belonging to the Family Astroscleridae (Demospongiae, Sclerospongiae, Ceratoporellidae).1) It is described as a red-orange, rock hard, 15cm spherical sponge, with a thin veneer of living tissue over an aragonite skeleton. The surface is porous and smooth, with 2cm diameter mounds. A reference sample preserved in ethanol has been deposited in the Harbor Branch Oceanographic Museum (catalog number 003:00989, DBMR number 24-VIII-85-1-053) and is available for taxonomic evaluation. Crude sponge sample (-100g) was diced and ground for three minutes in ethanol (40ml) three times. After the filtration and solvent removal in vacuum, the organic extract was fractionated on a column of polymeric resin, CG161 (-60ml) to generate 6 fractions using stepwise gradient of aqueous acetonitrile (ACN) (120ml of each 10%, 25%, 50%, 75%, 100% ACN, and 1:1 methanolEtOAc wash). All fractions were dried and yielded a total of 118mg of enriched material. Antifungal activity was observed in fraction 3 (45mg, 50% ACN elution). Fraction 3 was further fractionated on an HPLC semi-preparative

Structure Elucidation of a New Antifungal Sterol Sulfate, Sch 575867, from a Deep-Water Marine Sponge(Family: Astroscleridae).