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We performed experiments to screen for neutral sphingomyelinase inhibitors using rat brain microsomes as an enzyme source. Among more than 10,000 microbial extracts tested, a mycelial extract of Trichopeziza mollissima SANK 13892 exhibited potent inhibitory activity. The active compound, scyphostatin, was purified by a series of chromatographies. Scyphostatin inhibited the enzyme with an IC50 value of 1.0 microM.

journal of antibiotics

Scyphostatin, a Neutral Sphingomyelinase Inhibitor from a Discomycete, Trichopeziza mollissima: Taxonomy of the Producing Organism, Fermentation, Isolation, and Physico-chemical roperties.