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The culture broth (5 liters) was separated by filtration followed by concentration under reduced pressure (1 l iter) and extraction with ethyl acetate (3×1 liter). After drying over Na2SO4 the organic layer was evaporated under reduced pressure. The residue of 667mg was dissolved in 10ml hexane/isopropanol (7:3) and fracdonated on a silica gel column (Merck Silica gel 60, 0.1~0.063mm, 1.4×15cm) with the same solvent mixture as eluent. The bioactive fractions were determined

Alnumycin a New Naphthoquinone Antibiotic Produced by an Endophytic Streptomyces sp.