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liters of the production medium consisting of glucose 1.0%, soybeanmeal 1.0%,NaCl0.5% andCaCO3 0.1%, pH 7.0 before autoclaving. After 120 hours of cultivation at 30°C, the culture broth (4.0 liters) was centrifuged. The mycelium was extracted three times with MeOH,while the filtrate with ?7-BuOH.The active solvent extracts were combinedand evaporated to dryness in vacuo. The crude products were dissolved in a small amount ofMeOH,combined, filtered and precipitated with Me2CO-ether, 10: 1 (v/v). An amount of 2.60g crude powder was obtained after dryness in vacuo. A methanolic solution of 0.70g of the powder was chromatographed on a silica gel 60 (70~ 325mesh) column equilibrated with chloroform. The malonylniphimycin was eluted from the column by isocratic mode with a solvent mixture consisting of CHC13-MeOH-H20 175: 125:50 (v/v/v), lower phase.

Malonylniphimycin: Macrolide Antibiotic from Streptomyces hygroscopicus B-7: Physico-chemical Properties and Structure Elucidation.