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addition, radiolabeling studies done by Carter et al. have shown that the L-/?-lysine side chain of viomycin is derived from L-lysine7). Therefore, we reasoned that if the y-hydroxy-/?-lysine side chain of tuberactinomycin A was derived from L-/Mysine, then the addition of AEC would inhibit its formation and tuberactinamine A would accumulate as the final product. This hypothesis was demonstrated experimentally. To test the effect of AECaddition on the production profile of S. griseoverticillatus var. tuberadieus NRRL 3482, this strain was patched onto YPD agar (1% Difco yeast extract, 1% Difco Bacto peptone, 0.5% dextrose, 1% MOPS, and 1.7% Difco Bacto agar, pH 7.0) from a spore stock stored in 20% glycerol at -20°C. The patch was grown at 28°C for 5 days. After 5 days, a 6mmdiameter plug of the patch was inoculated into 30ml seed medium (2% soybean meal, 1%corn meal, 2% corn steep liquor, 0.6% NaNO3, and 0.02%

Production of Tuberactinamine A by Streptomyces griseoverticillatus var. tuberacticus NRRL 3482 Fed with (S)-2-Aminoethyl-L-cysteine.