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androstane skeleton with a keto-enol tautomerism at the cyclopentane ring. Wecarried out further isolation for PFTase inhibitors from the cultured broth of strain FO-3929 and found andrastin D (4, Fig. 1). In this paper, fermentation, isolation, structure elucidation, and biological properties of 4 are described. A stock culture of strain FO-3929 was inoculated into six 500-ml Erlenmeyer flasks containing 100 ml of a seed medium consisting of glucose 2.0%, yeast extract (Oriental Yeast Co.) 0.2%, MgSO4-7H2O 0.05%,

Andrastin D, Novel Protein Farnesyltransferase Inhibitor Produced by Penicillium sp. FO-3929.