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hydrogenase, thus blocking the biosynthesis of threonine, isoleucine and methionine2). Because these are essential dietary amino acids in animals, HONis potentially useful as a nontoxic antimicrobial drug. In addition, it may have value as a chiral starting material for the synthesis of more complex pharmaceutical agents. Analyses of HONenriched from isotopically labelled precursors have shown that C-l to C-4 are derived from aspartate, and C-5 from the methyl carbon of acetate40, but the

Mutants of Streptomyces akiyoshiensis Blocked in 5-Hydroxy-4-oxonorvaline Production.