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Pyralomicin 2a (5) -H -Cl -CH3 -CH3 Pyralomicin 2b (6) -H -CH3 -Cl -CH3 Pyralomicin2c(7) -H -Cl -CH3 -H Chemistry, Tokyo, Japan, produced new antibiotics, pyralomicins (1~7, Fig. 1). In this paper we report the production, isolation, physico-chemical properties and biological properties of 1 ~7. A slant culture of the pyralomicins-producing organism was inoculated into a 500-ml Erlenmyer flask containing 110ml of a seed medium consisting of galactose 2.0%, dextrin 2.0%, Bacto-soytone 1.0%, corn steep liquor 0.5%, (NH4)2SO4 0.2%, CaCO3 0.2% and a drop of silicon oil (adjusted to pH 7.4 before sterilization). The inoculated mediumwas incubated at 27°C for 72 hours on a rotary shaker. This pre-seed culture was transferred to two 30-literjar fernrenters each containing 12 liters of the same medium above described.

Pyralomicins, New Antibiotics from Actinomadura spiralis.