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A total of 76 mCi of 14C LL-F28249-alpha, nemadectin (1), having a specific activity of 35.2 microCi/mg was isolated from a fermentation using a mixture of approximately 600 mCi of 14C carboxyl labeled acetate, propionate and isobutyrate. Nemadectin was used to synthesize carbon-14 labeled moxidectin which is being developed as a highly efficient ectoparasitic anthelminth. The labeled positions were determined by 13C NMR analysis of 13C nemadectin which was obtained by similar incorporation of 13C carboxyl labeled acetate, propionate and isobutyrate.

Incorporation of carbon-14 in the biosynthesis of the macrolide antibiotic, IL-F28249-.ALPHA..