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CONTENTS Introduction Whyand HowCompanies Screen Microbial Metabolites The Return on Investment Organizational Issues Technical Aspects of the Laboratory Operations Optimizing Secondary Metabolite Diversity 1. Diversity and Novelty of Microorganisms 2. Fermentation of Microorganisms 3. Preparation of Fermentation Samples for Screening Screening Strategies and Tactics 1. Characteristics of Suitable Assays 2. Identification of Lead Cultures Dereplication Conclusions Acknowledgements References 535

Screening microbial metabolites for new drugs. Theoretical and practical issues.