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ionization to obtain mass spectral information on polyene macrolides, relatively little has been reported on the use of softer forms of chemical ionization such as direct liquid introduction (DLI). EI-MS of polyenes requires prior chemical derivatization of the many hydroxyl groups present in these molecules if high masses are to be seen. FABand FD have been found to give molecular ions and some useful high mass fragments with underivatized polyenes, but have been principally applied to pure compounds: coupling of FAB-MS with chromatography is

Direct liquid introduction LC-MS of polyene macrolide antibiotics: Comment on the structure of filipin II.