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The 1H and 13C NMR spectra of validamycin A have been assigned by use of 2D homonuclear correlation spectroscopy for protons, and off-resonance decoupling, single frequency off-resonance decoupling, and comparison studies with model compounds for carbons. Effects of pH on carbon chemical shifts have also been studied.

Biosynthetic studies on validamycins. I 1H and 13C NMR assignments of validamycin A.