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tion of 1 and the coproduced 6-deoxyilludin S (2). Agar slant cultures of P. japonicus ATCC 20195 were used to inoculate seed flasks containing 50-ml of a medium consisting of peptone (Kyokutou) 5 g, yeast extract 5 g, glucose 10 g, vegetative juice (V-8) 50ml, CaCO3 3 g and malt extract 2g per liter of deionized water. The inoculum was cultivated at 25°C for 2 days and added at the rate of 5% to the fermentation mediumconsisting of sucrose 50 g, soybean meal 20g, CaCO3 5g, KH2PO4 0.5g, MgSO4 7H2O 0.5 g and antiform agents LG-109 (Asahi Denka Kogyo) and KM-70 (Shinetsu

6-Deoxyilludin M, a new antitumor antibiotic: Fermentation, isolation and structural identification.