English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Plasmid-free strains of Streptomyces kasugaensis MB273 were isolated. In mating experiments S. kasugaensis MB273 was found to cause a lethal zygosis (pock) phenotype in a plasmid-free host. The pock-forming plasmids were identified as either pSK1* or pSK2* on the basis of their endonuclease cleavage-sites. The strain carrying pSK1* was found to induce pocks on the strain bearing pSK2*, and vice versa. The endonuclease cleavage-sites in pSK1* and pSK2* that were nonessential for pock formation were determined in deletion or insertion derivatives. The single sites for Bcl I and Sal I in pSK1* and for Bgl II in pSK2*, respectively, could be useful for DNA cloning without destroying pock-forming ability. Protoplasts of S. kasugaensis MB273-derivatives prepared in stationary phase of mycelial growth were competent for transformation, however, regeneration frequencies decreased during this phase.

journal of antibiotics

Plasmids of Streptomyces kasugaensis MB273: Their pock formation, their dispensable endonuclease cleavage sites for pock formation, and transformation of S. kasugaensis MB273 by them.