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The extent of the binding of beta-lactam antibiotics to 100,000 X g supernatant fluid of rabbit liver homogenates was investigated. Urinary excretion tended to decrease as the extent of the binding to 100,000 X g supernatant fluid increased. Predicting urinary excretion was achieved using the binding to 100,000 X g supernatant fluid and other physicochemical factors. A good coincidence resulted from multiple regression analysis. [14C]Benzylpenicillin binding proteins in rabbit liver were investigated and revealed that the affinity of cefoperazone was much stronger than that of cefazolin.

journal of antibiotics

Studies on protein binding of antibiotics. IV. Effect of the binding of drug to 100,000*g supernatant fluid of rabbit liver homogenates on urinary excretion.