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Methods have been developed for preparation of 4-N-arenesulfonylfortimicins B and 4-N-alkanesulfonylfortimicins B from derivatives of fortimicin B-1,5-carbamate. 4-N-(2-Aminoethanesulfonyl)fortimicin B and 4-N-(p-aminobenzenesulfonyl)fortimicin B have been prepared and found to be devoid of antibacterial activity.

4-N-(2-aminoethanesulfonyl)fortimicin B and 4-N-(p-aminobenzenesulfonyl)fortimicin B.