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Sir: Strain KM-6054, an actinomycete strain isolated from a soil sample collected at Setagayaku, Tokyo, has been reported to produce a new antibiotic, setamycin, showing activity against some fungi, trichomonads and Gram-positive bacteria.') In the course of taxonomic study, we found that the mycelia of strain KM-6054 contains both LL-diaminopimelic acid (DAP) and its meso-isomer, although this strain may be thought to be a strain of genus Streptomyces from the morphology, cultural characteristics, etc. It was also found that when DAP analysis was carried out separately on aerial and vegetative mycelia grown on an agar medium, most of DAP contained in aerial mycelia is LL-isomer while that in vegetative mycelia is meso-isomer (Fig. 1). There is no report concerning actinomycete strains which exhibit the above characteristics. The present communication deals with taxonomic properties of strain KM-6054. The procedures described by SHiRLING and GOTTLIEB') were followed for morphology and cultural and physiological characteristics. Strain KM-6054 is well developed on both synthetic and complex media. No fragmentation of the vegetative mycelium is observed (Plate 1). The aerial mycelium, morphology of which is classified in the section Rectus flexibilis, has more than 20 spores per chain (Plate 2). The spores have smooth surfaces and are cylindrical in shape and 0.7 x 0.8 1.6 tc in size. No sporangium and zoospore are observed. The colony of strain KM-6054 is leathery. Vegetative mass color shows yellow or brown. White aerial mycelia are abundantly formed on yeast extract-malt extract agar, oatmeal agar and inorganic salts starch agar. The strain produces a small amount of yellow soluble pigment in inorganic salts starch agar. The formation of melanoid pigment is not observed. The culture grows at 2237°C. The morphology described above and the gaschromatogram pattern of cellular fatty acids by

Type of diaminopimelic acid different in aerial and vegetative mycelia of setamycin-producing Actinomycete KM-6054.