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A mutant strain derived from Nocardia mediterranei N813 was found to accumulate a number of novel ansamycins structurally related to the protostreptovaricins and to rifamycin W. One of the main components of this ansamycin complex, protorifamycin I (8-deoxyrifamycin W), was purified and identified by means of chemical and spectroscopic methods.

Early intermediates in the biosynthesis of ansamycins. I. Isolation and identification of protorifamycin I.