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Thiocillins I and II were isolated from the culture broth of Bacillus cereus G-15, and thiocillins II and III from that of Bacillus badius AR-91. Also, the former two were probably produced by Bacillus megatherium I-13. These antibiotics active against Gram-positive bacteria are soluble in a mixture of chloroform and methanol, show characteristic ultraviolet absorptions (maxima at ca. 275 nm and ca. 348 nm), and contain a high content of sulfur, as much as approximately 15%. They are related to each other and also to micrococcin P, but differentiated by chromatographic behaviours.

Studies on antibiotics from the genus Bacillus. VIII. Isolation of three new antibiotics, thiocillins I, II and III, related to micrococcin P.