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A new aminoglycoside antibiotic XK-62-2 (Sagamicin) produced by Micromonospora sagamiensis var. nonreducans nov. sp. MK 62 was isolated from its fermentation beer by use of a cationic exchange resin and silica-gel column chromatography. The purified antibiotic showed a close similarity to gentamicin C complex in physical, chemical, and antimicrobial properties, but a significant difference from gentamicin in its more remarkable activity against some Pseudomonas aeruginosa strains resistant to gentamicin C1a.

A new antibiotic XK-62-2 (Sagamicin). I. Isolation, physicochemical and antibacterial properties.