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Arpa Sari Cucelik Hastaligi, tum dunyada bugday uretim alanlarinda en yaygin ve ekonomik kayip olusturan viral hastaliklardan biridir. Bu hastaliga Luteoviridae familyasinda yer alan Barley yellow dwarf virus turlerinden (BYDVs) herhangi biri neden olmaktadir. Bu calismada, Afyonkarahisar ve Isparta illerindeki bugday uretim alanlarinda Barley yellow dwarf virus PAV (BYDV-PAV) ve Barley yellow dwarf virus MAV (BYDV-MAV)’in belirlenmesi amaciyla 2016 yili bugday uretim doneminde virus belirtisi gosteren 94 adet bitki ornegi toplanmistir. BYDV-PAV ve BYDV-MAV’in varligi DAS-ELISA (Double antibody sandwich enzyme linked immunosorbent assay) test yontemi ile belirlenmistir. DAS-ELISA test sonuclarina gore 94 ornegin 68 adedinde % 72.3 oraninda BYDV-PAV ve 2 ornekte ise % 2.1 oraninda BYDV-MAV tekli enfeksiyonlari belirlenmistir. Orneklerin sadece 4 adedinde % 4.2 oraninda her iki virusun karisik enfeksiyonu tespit edilmistir.

Afyonkarahisar ve Isparta illerindeki buğday üretim alanlarında Barley yellow dwarf virus-PAV ve Barley yellow dwarf virus-MAV’ ın belirlenmesi