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shown to natively internalize such modified DNA The basic parameters for this DNA probe internalization by the murine Krebs-2 tumor cells were characterized Upon internalization, the fragments of DNA undergo partial and nonuniform hydrolysis of 3′ ends followed by ligation into a ring The degree of hydrolysis, assessed by sequencing several clones with the insertion of specific PCR product, was 30-60 nucleotides Conclusions: Results of the current investigation suggest the possibility to use FapdC-modified DNA fragments for further analysis of both membrane-associated and intracellular factors mediating the internalization of eDNA by Krebs-2 cells Capability of Fap-dCDNA to impair the NER process presumes their possible applicability in antitumor therapy

A CRISPR view of the genome in living cells: cell cycle and genomic distance dependent dynamics of chromosomal loci