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Different conditions of seeding human bone marrow stromal cells (SC) into polymer sponges based on macroporous agarose cryogel (MACG) were investigated. Three methods of cell seeding into MACG were used: co-incubation with permanent stirring, intensive shaking and creation of negative pressure. The results obtained show a considerable potential of macroporous agarose cryogel sponges as a three-dimensional carrier for cultivating stromal cells at different methods of cell seeding.

Choice of conditions of human bone marrow stromal cells seeding into polymer macroporus sponges