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The process of affinity maturation in the course of humoral immune response of BALB/c mice against horse muscle myoglobin was studied. For this purpose we used the method of determination of the affinity constants for highand low-affinity antibodies, present in the mixture. Application of this method allowed us to determine the main characteristics of humoral immune response change in the course of the immune response against T-dependent protein antigen. It was shown that the affinity of antibodies, their quantity, and the proportion of high-affinity specific antibodies gradually increased during immune response development.

Affinity maturation of antibodies in the course of humoral immune response