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Cal lus tis sues from in di vid ual maize plants of line P346 and de rived from it through in vi tro tis sue cul ture of somaclonal vari ants show ing in creased re gen er a tion ca pac ity have been an a lyzed by RAPD. As early as af ter two months in cul ture maize calli ex hib ited ge nome changes but fol low ing four months their level de creased al most twice. The emer gence of homotypic DNA changes in calli ob tained from dif fer ent plants of in di vid ual lines was reg is tered thus sug gest ing the ex is tence of vari abil ity hot-spots within the maize ge nome. The somaclonal lines were shown to vary by the level of the ge netic changes in cul ture in vi tro.

Genomic variability in maize callus cultures of lines P346 and its derivative somaclonal lines