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The recombinant plasmids containing the genomic human apoA-1 gene under the transcriptional control of hybrid promoters, namely, the cytomegalovirus immediate early enhancer/chicken ?-actin promoter and first intron (CAG promoter) and cytomegalovirus immediate early enhancer/promoter with intron A (hCMVintronA promoter) have been constructed. The transient expression of human apoA-1 gene has been studied in CHO-K1 cells using designed and pTRapo vectors. The expression of the transgene for transfected with pTRapo and pTRapochCMVintronA CHO-K1 cells have been shown.

Study of transient expression of human apoA-1 gene under control of various promoters in CHO-K1 cells