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A new system for fluorescent 5'-end labelling of oligonucleoti des with cyanine dyes is proposed. Reaction of primary amine with pyrylium salt was used for the efficient conjugation of monomethyne pyrylium cyanine dye with 5'-aminoalkyl oligonucleotide with simultaneous conversion of the «brightless» pyrylium dye Cyan 39 into «bright» pyridinium one Cyan 40.

biopolymers and cell

Interaction of cyanine dyes with nucleic acids. 4. Efficient 5'-fluorescent labelling of oligonucleotides with monomethyne pyrylium cyanine dye, Cyan 39