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Aim. To investigate 24-epibrassinolide influence on the maize seedlings 9- and 13-lipoxygenases activity (9- and 13-LOX) under normal conditions (25o C) and cold stress (5o C). Methods. LOX activity was measured after treatment of seedlings with 0.01 and 1 ìM 24-epibrassinolide. The enzymes were extracted from maize seedlings with 0.1 M sodium acetate (pH 4.5) buffer, supplemented with a non-ionic detergent (0.1 % Brij-99) and EDTA (0.1 mM). The 9- and 13-LOX activities were determined spectrophotometrically at 234 nm using linoleic acid as substrate at pH 6.0 and 7.0 in the presence or absence of 0.02 % Lubrol PX. Results. 3-6-fold increase in LOX activity of 24-epibrassinolide-treated seedlings was demonstrated under normal conditions. Cold stress in the presence of 1µM 24-epibrassinolide enhances the activities of 9- and 13-LOX by 4 and 10 times, respectively. Conclusions. The results obtained enlarge our understanding of possible pathways of LOX metabolites involvement in the formation of cell response to brassinosteroids

Effect of 24-epibrassinolide on lipoxygenase activity in maize seedlings under cold stress