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Objectives : Allergy is an immune dysfunction caused by degranulation from m ast cells in the early phase of allergic disease. The purpose of this study was to investigate the anti-allergic effect of fermented Angelicae gigantis Radix in human mast cell line, HMC-1.Method : The Angelicae gigantis Radix was fermented by Lactobacillus acidophilus. The cell toxicity of fermented Angelicae gigantis Radix(FAGR) was determined by MTT assay. The release of β-hexosamini dase from HMC-1 stimulated by phorbol-12-myristate 13-acetate (PMA) plus A23187 was determined by β-hexosaminidase assay. Also, the concentrations of cytokines (i nterleukin-1β, -6, -8 and tumor necrosis factor-alpha) were measured by enzyme-linked immunosorbent assa y. The gene expression of COX-2 from HMC-1 stimulated by phorbol-12-myristate 13-acetate (PMA) plus A23187 was determined by reverse transcription polymerase chain reaction. The release of histami ne on substance P-stimulated HMC-1 was measured by histamine assay.Result : The FAGR suppressed the release of β-hexosaminidase, a marker o f degranulation, from HMC-1 stimulated by PMA plus A23187. The FAGR inhibited the productio n of interleukin-1β, -6, -8 and tumor necrosis factor-alpha. The FAGR inhibited the expression of COX -2 mRNA. The FAGR suppressed the release of histamine on substance P-stimulated HMC-1.Conclusion : These results provide that FAGR may be beneficial in the treatment of allergic inflammatory disease.Key words : ferment, Angelicae gigantis radix, cytokines, allergy

Anti-allergic Effect of the Fermented Angelicae Gigantis Radix in Human Mast Cell Line HMC-1