English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We have developed a genetic marker that can identify a registered variety of mat rush in Japan. A vegetatively propagated plant, mat rush is cultivated and used as the material for the surface layer of tatami mats in Japan. Because it has been difficult to detect DNA polymorphism among mat rush cultivars, we applied restriction landmark genome scanning (RLGS) to discriminate mat rush cultivars. RLGS is a genome analysis technique that can detect many DNA polymorphisms as spots separated by two-dimensional electrophoresis. By cloning the DNA of spots specific to the superior mat rush cultivar ‘Hinomidori’ detected by RLGS, we developed a sequence-tagged site (STS) marker for polymerase chain reaction (PCR) analyses. This STS marker makes it possible to distinguish ‘Hinomidori’ specifically from other mat rush cultivars. The strategy of developing the STS marker in this study is applicable to other vegetatively propagated plants that are characterized by difficult DNA polymorphism detection. Discipline: Biotechnology Additional key words: DNA polymorphism, PCR, RLGS, spot cloning, vegetatively propagated plant *Corresponding author: e-mail okuizumi@affrc.go.jp Received 24 September 2015; accepted 19 April 2016. Introduction Mat rush (Juncus effusus) is a perennial plant distributed widely throughout warm temperate zones in Eurasia and the American continents. The plants grow naturally in Asia, including in Japan, China, Taiwan, and Korea. J. effusus is bred and cultivated to make tatami omote, rug mats, wicks for oil lamps, and industrial art objects. Breeding is mainly based on bud mutations in the crossing parent plants. Consequently, the genetic diversity among the domesticated groups is considered to be low. There have been frequent cases of a superior mat rush cultivar (‘Hinomidori’) being illegally imported back to Japan for use as a raw material to make tatami omote, mats, and sandals. ‘Hinomidori’ is the first cultivar in mat rush improved by artificial crossing, and the products exhibit more attractive surfaces owing to thin traces without flower buds (Nakazawa et al. 1999). It was registered in 2001 and sold for a high price (Noguchi & Kako 2006). Given the high quality of ‘Hinomidori,’ cultivar identification methods have become more important. However, differentiation by visual inspection is difficult. Therefore, a technique for DNA cultivar identification is required for the protection of intellectual rights.

A Sequence-tagged Site Marker for Identifying the Japanese Mat Rush (<i>Juncus effusus</i>) Cultivar ‘Hinomidori’